mesenchymal stem cell characterize
One of the most important aspects of the manufacturing of cell-based therapy products is the definition and measurement of cell characteristics. This can be broken down into identity, potency, purity, and safety, which must all be considered during product development. With the help of our well-established technologies and years of experience, Creative Biolabs can provide phenotypic, genetic, and functional assessments of your MSC population of interest using various techniques and tools. We adapt to your requirements by providing either individual service modules or a fully comprehensive service package.
The Characterization of MSCs
Due to the population heterogeneity of MSC cultures resulting from factors such as different tissue derivations, diverse isolation protocols, and various growth conditions, a common guideline for MSC preparations is required to be defined to characterize MSC populations. The basic characteristics and minimal criteria of MSCs were declared in 2006 by the International Society for Cellular Therapy (ISCT) (Fig. 1). Since then, additional markers have been identified and widely accepted as reliable MSC surface markers, such as STRO-1, CD271, CD348, CD200, and CD105. Studies have also found that functional and phenotypic differences may exist across tissue sources, culture conditions, and the extent of ex-vivo expansion. Thus, tissue-specific expression of different surface markers should be considered when describing MSCs derived from different sources.
